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| Bionatura |
Abstract:
| The genetic diversity of 4 R. leguminosarum bv. trifolii and R. leguminosarum bv. Phaseoli that was extracted from root nodes of Trifolium spp and Phaseolus vul- garis L cultivars circulated in Mosul soils were investigated. The bacteria strains under study show similar tolerance levels against some external factors. In the current study, using classical diagnosis methods, similarity and distance indices and DNA polymorphism were examined with the randomly amplified polymor- phic DNA. The isolates were distributed through genetic diversification into two main classes, including two main branches, Rl2 and the other sub-group was di- vided into Rl3, and the neighboring group involved Rl1 and Rl4. It is noted that individual plants and abiotic aspects were less affected than the Genetic factor on bacterial diversity. On the other hand, in multiplex-PCR reaction, Our outcomes revealed specific amplified for R. leguminosarum bv. trifolii with amplification products 419 bp and R. leguminosarum bv. Phaseoli with amplification products 362 bp. |