The current study was conducted in Misan Governorate to detect the parasite Sarcocystis spp. by both conventional and molecular methods and to identify the species of this genus in goats as intermediate hosts for multiple species of this parasite. Regarding to the traditional study, the rates of infection with the macroscopic type of Sarcocystis spp. were low and recorded 2% in esophageal samples only in goats. The sizes of macroscopic parasite cysts ranged between 1-10 mm in length and 2-6 mm in width. Infection with the microscopic type of parasite recorded high rates. When comparing the efficiency of the traditional methods used in our study to diagnose the infection of the microscopic type in all of the examined organs isolated from goats, which included the methods of digestion with pepsin, squeezing, trichinoscopy. It was found that the method of digestion with pepsin was the most efficient traditional method for diagnosing the microscopic type of parasite, followed by the trichinoscopy. While the squeezing method recorded the least efficient traditional method for diagnosing Sarcocystis spp. microscopically.
